{"id":4330,"date":"2022-03-30T14:01:09","date_gmt":"2022-03-30T06:01:09","guid":{"rendered":"http:\/\/hongyunqingxiao.com\/?p=4330"},"modified":"2023-11-09T23:13:08","modified_gmt":"2023-11-09T15:13:08","slug":"%e4%bd%93%e5%a4%96%e8%bd%ac%e5%bd%95%ef%bc%88ivt%ef%bc%89rna%e5%bd%a2%e5%bc%8f%e5%8f%8arnp%e5%bd%a2%e5%bc%8f%e7%9a%84%e9%94%90%e6%b9%83%e5%b0%94%e5%9f%ba%e5%9b%a0%e7%bc%96%e8%be%91%e6%8a%80","status":"publish","type":"post","link":"https:\/\/www.hongyunqingxiao.com\/?p=4330","title":{"rendered":"\u4f53\u5916\u8f6c\u5f55\uff08IVT\uff09RNA\u5f62\u5f0f\u53caRNP\u5f62\u5f0f\u7684\u9510\u6e43\u5c14\u00ae\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u5c55\u793a\u5bf9\u4eba\u6e90\u7ec6\u80de\u7684\u57fa\u56e0\u7f16\u8f91\u80fd\u529b\uff0c\u540c\u65f6\u5177\u5907\u8fdb\u4e00\u6b65\u5de5\u4e1a\u5316\u751f\u4ea7\u6f5c\u80fd\u3002"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"4330\" class=\"elementor elementor-4330\" 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class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u5b8c\u5168\u4eba\u6e90\u7684\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u2014\u9510\u6e43\u5c14<sup>\u00ae<\/sup>\uff08REPERE<sup>\u00ae<\/sup>\uff09\uff0c\u5df2\u5b9e\u73b0\u901a\u8fc7\u4e8e\u4f53\u5916\u8f6c\u5f55\u4ea7\u751fRNA\u5e76\u4e8e\u4f53\u5916\u7ed3\u5408ORF1p\u548cORF2p\u5f62\u6210RNP\u540e\u8f6c\u5165\u4eba\u6e90\u7ec6\u80de\u5e76\u5bf9\u5176\u8fdb\u884c\u57fa\u56e0\u7f16\u8f91\uff0c\u5c06\u5916\u6e90\u5e8f\u5217\u6709\u6548\u5e76\u51c6\u786e\u7684\u63d2\u5165\u81f3\u4eba\u6e90\u7ec6\u80de\u57fa\u56e0\u7ec4\u4e2d\u76f8\u5e94\u9776\u4f4d\u70b9\u5904\u3002<\/p><p>\u4f8b\uff1a\u4e00\u6bb5\u5916\u6e90\u6027\u5e8f\u5217\u53ca\u9776\u4f4d\u70b9\u7684\u4e0a\u4e0b\u6e38\u5e8f\u5217\u8fde\u63a5\u7ecf\u6539\u9020\u7684Alu\u5e8f\u5217\uff0c\u8be5\u5e8f\u5217\u5728\u4f53\u5916\u7ecfT7\u542f\u52a8\u5b50\u8f6c\u5f55\u4ea7\u751fRNA\u540e\uff0c\u4e8e\u4f53\u5916\u7ed3\u5408ORF1p\u53caORF2p\u540e\u5bfc\u5165Hela\u7ec6\u80de\uff0c\u901a\u8fc7\u9510\u6e43\u5c14<sup>\u00ae<\/sup>\uff08REPERE<sup>\u00ae<\/sup>\uff09\u6280\u672f\u5c06\u5176\u63d2\u5165\u81f3Hela\u7ec6\u80de\u7684<em>Lman1<\/em>\u57fa\u56e0\u4e2d\u7684\u9776\u4f4d\u70b9\u3002qPCR\u7ed3\u679c\u663e\u793a\uff1a<\/p><table width=\"622\"><tbody><tr><td width=\"140\">Ct\u503c<\/td><td width=\"57\">GAPDH<\/td><td width=\"63\">\u5f85\u63d2\u5165\u5e8f\u5217\u63d2\u5165<\/td><td width=\"94\">\u0394Ct<\/td><td width=\"102\">\u0394\u0394Ct\uff08\u5b9e\u9a8c\u7ec4\u0394Ct\u5e73\u5747\u503c-\u5bf9\u71671\u7ec4\u0394Ct\u5e73\u5747\u503c\uff09<\/td><td width=\"166\">\u62f7\u8d1d\u6570\u76f8\u5bf9\u91cf\uff082<sup>-\u0394\u0394Ct<\/sup>\uff09<\/td><\/tr><tr><td width=\"140\">\u5bf9\u71671\u7ec4\u677f1<\/td><td width=\"57\">26.23<\/td><td width=\"63\">N\/A<\/td><td width=\"94\">13.77<\/td><td width=\"102\">&#8211;<\/td><td width=\"166\">&#8211;<\/td><\/tr><tr><td width=\"140\">\u5bf9\u71671\u7ec4\u677f2<\/td><td width=\"57\">26.14<\/td><td width=\"63\">N\/A<\/td><td width=\"94\">13.86<\/td><td width=\"102\">&#8211;<\/td><td width=\"166\">&#8211;<\/td><\/tr><tr><td width=\"140\">\u5bf9\u71671\u7ec4\u677f3<\/td><td width=\"57\">25.80<\/td><td width=\"63\">N\/A<\/td><td width=\"94\">14.20<\/td><td width=\"102\">&#8211;<\/td><td width=\"166\">&#8211;<\/td><\/tr><tr><td width=\"140\">\u5bf9\u71671\u7ec4\u5e73\u5747\u503c<\/td><td width=\"57\">\u00a0<\/td><td width=\"63\">\u00a0<\/td><td width=\"94\">13.94\u00b10.19<\/td><td width=\"102\">0.00\u00b10.19<\/td><td width=\"166\">1.00\uff080.88-1.14\uff09<\/td><\/tr><tr><td width=\"140\">\u5b9e\u9a8c6\u7ec4\u677f1<\/td><td width=\"57\">25.72<\/td><td width=\"63\">26.45<\/td><td width=\"94\">0.73<\/td><td width=\"102\">\u00a0<\/td><td width=\"166\">\u00a0<\/td><\/tr><tr><td width=\"140\">\u5b9e\u9a8c6\u7ec4\u677f2<\/td><td width=\"57\">26.08<\/td><td width=\"63\">26.19<\/td><td width=\"94\">0.11<\/td><td width=\"102\">\u00a0<\/td><td width=\"166\">\u00a0<\/td><\/tr><tr><td width=\"140\">\u5b9e\u9a8c6\u7ec4\u677f3<\/td><td width=\"57\">26.33<\/td><td width=\"63\">26.59<\/td><td width=\"94\">0.26<\/td><td width=\"102\">\u00a0<\/td><td width=\"166\">\u00a0<\/td><\/tr><tr><td width=\"140\">\u5b9e\u9a8c6\u7ec4\u5e73\u5747\u503c<\/td><td width=\"57\">\u00a0<\/td><td width=\"63\">\u00a0<\/td><td width=\"94\">0.37\u00b10.26<\/td><td width=\"102\">-13.57\u00b10.26<\/td><td 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